Product Info Summary
SKU: | PB9038 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-MMP7 Antibody Picoband™
SKU/Catalog Number
PB9038
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MMP7 Antibody Picoband™ catalog # PB9038. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MMP7 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9038)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse MMP7 recombinant protein (Position: Y95-L264). Mouse MMP7 shares 71% and 90% amino acid (aa) sequences identity with human and rat MMP7, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9038 is reactive to MMP7 in Mouse, Rat
Applications
PB9038 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
30 kDa
Calculated molecular weight
29.677kDa
Background of MMP-7
Matrix metalloproteinase-7 (MMP-7) previously called putative metalloproteinase I (PUMP1) or matrilysin. The MMP-7 gene has been identified through studies of collagenase-related connective-tissue-degrading metalloproteinases produced by human tumors. The MMP-7 protein has 267 amino acids and is significantly shorter than stromelysin or collagenase (477 and 469 amino acids, respectively). Matrix metalloproteinases play a crucial role in tumor invasion and metastasis. MMP-7, a member of the matrix metalloproteinase family, is structurally different from the other matrix metalloproteinases by virtue of the absence of a conserved COOH-terminal protein domain. In addition, MMP-7 mRNA is regulated in a specific and distinct manner in normal and malignant tissues. MMP-7 has been shown to correlate with nodal or distant metastasis in colorectal carcinomas, however, its implication in early invasive colorectal carcinomas has not been determined. MMP-7 is also a mediator of pulmonary fibrosis and a potential therapeutic target. The standard product used in this kit is recombinant human MMP-7, consisting of 250 amino acids with the molecular mass of 28KDa. The detected MMP-7 includes zymogen and active enzyme.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA , 0.1-0.5μg/ml, Mouse, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Mouse
Validation Images & Assay Conditions
Click image to see more details
Figure 1. IHC analysis of MMP7 using anti-MMP7 antibody (PB9038).
MMP7 was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MMP7 Antibody (PB9038) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 2. IHC analysis of MMP7 using anti-MMP7 antibody (PB9038).
MMP7 was detected in paraffin-embedded section of rat intestines tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MMP7 Antibody (PB9038) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. Western blot analysis of MMP7 using anti-MMP7 antibody (PB9038).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat Brain Tissue Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP7 antigen affinity purified polyclonal antibody (Catalog # PB9038) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP7 at approximately 30KD. The expected band size for MMP7 is at 30KD.
Protein Target Info & Infographic
Gene/Protein Information For MMP7 (Source: Uniprot.org, NCBI)
Gene Name
MMP7
Full Name
Matrilysin
Weight
29.677kDa
Superfamily
peptidase M10A family
Alternative Names
EC 3.4.24; EC 3.4.24.23; Matrilysin; matrin; matrix metallopeptidase 7 (matrilysin, uterine); matrix metalloproteinase 7 (matrilysin, uterine); Matrix metalloproteinase-7; MMP7; MMP-7; MPSL1; Pump-1 protease; PUMP1; PUMP-1; uterine matrilysin; Uterine metalloproteinase MMP7 MMP-7, MPSL1, PUMP-1 matrix metallopeptidase 7 matrilysin|matrin|matrix metallopeptidase 7 (matrilysin, uterine)|matrix metalloproteinase 7 (matrilysin, uterine)|matrix metalloproteinase-7|pump-1 protease|uterine matrilysin|uterine metalloproteinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MMP7, check out the MMP7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MMP7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MMP7 Antibody Picoband™ (PB9038)
Hello CJ!
PB9038 has been cited in 9 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Herbal medicine Gan‑fu‑kang downregulates Wnt/Ca2+ signaling to attenuate liver fibrogenesis in vitro and in vivo
Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells
Hydrogen sulfide reduced renal tissue fibrosis by regulating autophagy in diabetic rats
Glycyrrhizic acid alleviates bleomycin-induced pulmonary fibrosis in rats
Sun Y, Chen Y, Li S, Lei Y, Xu D, Jiang N, Zhang Y, Cao J, Ke Z. Int J Nanomedicine. 2017 Aug 31;12:6399-6412. doi: 10.2147/IJN.S144033. eCollection 2017. NanoVelcro-captured CTC number concomitant with enhanced serum levels of MMP7 and MMP9 enabl...
Xu Q, Xu HX, Li JP, Wang S, Fu Z, Jia J, Wang L, Zhu ZF, Lu R, Yao Z. Oncotarget. 2017 Mar 7;8(10):16972-16987. doi: 10.18632/oncotarget.15216. Growth differentiation factor 15 induces growth and metastasis of human liver cancer stem-like cells vi...
Yang B, Gao J, Rao Z, Zhang B, Ouyang W, Yang C. Ultrastruct Pathol. 2011 Dec;35(6):256-9. Doi: 10.3109/01913123.2011.610564. Epub 2011 Oct 7. Antisense Oligonucleotide Targeting Matrix Metalloproteinase-7 (Mmp-7) Changes The Ultrastructure Of Hum...
Pan Fy, Zhang Sz, Xu N, Meng Fl, Zhang Hx, Xue B, Han X, Li Cj. Histochem Cell Biol. 2010 Sep;134(3):285-95. Doi: 10.1007/S00418-010-0729-3. Epub 2010 Jul 31. Beta-Catenin Signaling Involves Hgf-Enhanced Hepg2 Scattering Through Activating Mmp-7 T...
Yang Y, Shi H, Li X, Yi Y. Oncol Rep. 2011 Jan;25(1):259-65. Effects Of Shrna Targeting Maspin On Invasion Of Gastric Carcinoma Sgc7901 Cell Line.
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-MMP7 Antibody Picoband™
Question
We are currently using anti-MMP7 antibody PB9038 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
B. Edwards
Verified customer
Asked: 2015-11-11
Answer
The anti-MMP7 antibody (PB9038) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-11-11